Assay of Dihydroartemisinin by Iodometric Titration

Contenu principal de l'article

NIJOPHASR
Attih E. E.
Essien E. E.
Eseyin O. A.
Oladimeji H. O.
Igboasoiyi A. C.

Résumé

The iodometric titration method is developed for the determination of dihydroartemisinin in
tablets and in bulk powders. The method is based on the redox reaction between the drug and
potassium iodide in acid condition. Iodine is generated in situ by this reaction. The liberated iodine
is then titrated with standardised sodium thiosulphate reagent with boiled starch as indicator. It
was found that the percentage content of active ingredient per tablet was 98% which falls within
the International pharmacopoeia standard (90-110%). This titrimetric method is based on a 1:1
reaction stoichiometry of dihydroartemisinin: iodine. This method is applicable over the range of 5-
70mg and was used to assay other popular brands of dihydroartemisinin like Alaxin, Cotecxin and
Santexin tablets sold in drug outlets in Uyo, Nigeria. The proposed method was found to be very
useful when applied to these tablet preparations with mean recoveries of 98% to 104%.The method
was evaluated for precision, accuracy and recovery studies using standard addition technique,with
the relative error of < 2%, RSD < 3% and a coefficient of variation < 3%. The results of this method
was statistically compared with the reference method by applying the students t-test and F-test at
95% confidence level ( t = 2.77 and F = 6.33) the calculated t and F values did not exceed the
tabulated values at 4 degrees of freedom showing no significant difference between the proposed
method and the reference method in terms of precision and accuracy.

Téléchargements

Les données relatives au téléchargement ne sont pas encore disponibles.

Renseignements sur l'article

Comment citer
NIJOPHASR, E. E., A., E. E., E., O. A., E. ., H. O., O. ., & A. C., I. (2020). Assay of Dihydroartemisinin by Iodometric Titration. Nigerian Journal of Pharmaceutical and Applied Science Research, 1(2). Consulté à l’adresse http://mail.nijophasr.net/index.php/nijophasr/article/view/18
Rubrique
Articles

Références

Burke E., Deasy J., Hasson R., Mc-Corrmarck R., Randhana V., and Walsh(2003)Antimalarial drugs from nature, qinghaosu/Artemisinin.

http.tcd.ie/tsnj/2003/antimal.htm

Coker,H.A. B., Chukwuani, C. M. and Aina. N. D. (2001). The Malaria Scourge, concepts in disease management. Nigerian Journal of Pharmacy, 32. 19 - 47.

China Co-operative Research Group on Qinghaosu and its Derivatives as Antimalarials(1982) J.Tradit.Chin.Med. 2:9

Gabriels M. and Plaizier-Vercammen J. (2004) Development of a reverse phase thin layer chromatographic method for

artemisinin and its derivatives. J. Chromatogr/Sci. 42:341.

Green M. D, Mount D. L, Wirt R. A and White N. J (2000) A colorimetric field method to assess the authenticity of drugs sold as the

antimalarial Artesunate,JPharm.Biomed. Anal.24 ; 65 – 70.

Green M. D., Mount D. L. et al (2001). Authentication of arthemether, artesunate and dihydroartemisinin antimalarial tablets

using a simple colorimeter method.Tropical Medicine and International Health; T M & I H 6(12): 980-982.

Klayman D. L. (1985) QINGHAOSU (ARTEMISININ): an antimalarial drug from China. Science 228:1049.

Kopps S. (2003). Monograph for Antimalarial Drugs, artemisinin and derivatives. International pharmacopoeia Vol.

3rd edition.WHO CH 22 N Geneva 27, Switzerland.

Li Y., Yu P-L,Chen Y-X , Li L-Q, Gai Y. Z., Wang D. S. and Zheng Y-P(1981) Studies on analogs of artemisinin,1. Synthesis of

ethers carboxylic esters and carbonates of dihydroartemisinin. ActaPharmaceutic a Sinica.16:429.

Liu J-M, Ni M-Y, Fan J-F, Tu Y-Y, Wu Z-H and Zhou W-S (1979)Structure and reactions of Artemisinin. ActaChimicaSinica, 37:129.

Lin A. J., Klayman D. L. and Milhous W. K. (1987) Antimalarial of new water soluble dihydroartemisinin derivatives.J. Med. Chem. 30: 2147.

Meshnic S. R., Taylor T. E., and Kamchonwongpaisain S. (1996) Artemisinin and the endoperoxides from herbal remedy to targeted

chemotherapy.Microbiol. Rev. 60: 169

Na-Bangchang K., Congpuong K., Hung L. N., Molunto P. and Karbwang J. (1998) Simple high-performance liquid chromatographic method with electrochemical detection for simultaneous determination of artesunate and dihydroartemisinin in biological fluids. J. Chromatogr. B. Biomed. Science Appl. 708: 201.

Naik H., Murry D. J. Kirsch L. E. and Fleckenstein L. (2005) Development and validation of high performance liquid chromatography – mass spectroscopy assay for determination of artesunate and dihydroartemisinin in human plasma J. Chromatogr. B. Analyt Technol. Biomed. Life Sci. 816: 233.

Newton P. N., Dondorp et al (2003) Counterfeit artesunateantimalarials in South East Asia. Lancet 362(9378):169.

Robert A. B., Dechy-Cabaret O. and Meunier B. (2001). From Classical antimalarial drugs to new compounds based on the

mechanism of action of artemisinin. Journal of Pure and Applied Chemistry, 73(73): 1173-1188.

Sanjeev Krishna, Ann-CatrinUhlemann, Richard K. Haynes. (2004): Artemisinin: Mechanism of action and potential for resistance. Journal of Drugs Resistance Update. 7(10) 233-244.