Determination of Dihydroartemisinin In Bulk And Pharmaceutical Formulations By Redox Titrations And UV – Spectrophotometry Using Potassium Permanganate As Oxidimetric Reagent
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Résumé
Two new methods are proposed for the determination of dihydroartemisinin (DHA) in bulk and pharmaceutical formulations. Method A Titrimetry, based on the redox reaction of DHA and potassium permanganate in acid medium. Method B, UV-Spectrophotometry based on the redox reaction of DHA and potassium permanganate in acid medium giving a product which absorbs UV light at 520nm. In both methods the amount of potassium permanganate used is proportional to the amount of DHA. Experimental conditions for good linearity, sensitivity, specificity accuracy and precision were optimized. In method A (titrimetry) the calculations are based on a 2:5 (DHA: KMnO4) reaction stoichiometry) and this method is applicable over the practical range of 5.0 – 20mgml-1. Method B obeys Beer’s law. The calibration curve generated is linear with correlation
coefficient of (r) of 0.9998 (n = 10). The molar absoptivity is 2.27 x103 Lmol cm-1 and sandell sensitivity of 0.126?gcm-2 The limit of detection (LOD) and limit of quantification (LOQ) were determined as per the current ICH guidelines and found to be 0.88 and 2.66?gml-1 respectively. Accuracy and precision of both method were determined using intra and inter day variations at three different concentration level of DHA the relative standard deviation (RSD %) were <2.00 and <2.5 respectively. The two methods were used to assay DHA in 4 brands of tablets formulation procured locally in Uyo. South-South, Nigeria at three different concentration level, t and F value of <2.40 and <3.0 at 95% confidence level and 5 degree of freedom. These values are lower than tabulated t and F values. The methods were statistically compared with an official method with congruent results. There was no interference from common pharmaceutical excipients. Recovery study was also performed
via standard addition procedure with excellent recoveries.
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