Antimicrobial Properties and Molecular Docking Studies of Medicinal Compounds from Moringa Oleifera and Zingiber Officinale against Escherichia coli
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Background:Infectious diseases are one of the major causes of more than half of all human deaths.
Objectives:The persistent occurrences of drug resistant bacteria to conventional antimicrobial agent justified the need to search for alternative drugs especially from plant.
Methods: in vitro antibacterial activity of M. oleifera leaves and Z. officinale rhizomes on E. coli were investigated, Broth dilution method was used to determine the Minimum inhibitory concentration (MIC). Molecular docking studies were performed with Maestro program. Lead compounds were identified based on their docking scores and mechanism of action of the compounds on the target organism.
Results: Moringa and Ginger respectively were found to inhibit the growth of the bacteria at concentrations 400mg/ml and 200mg/ml only. The MIC for Moringa and Ginger was 200 mg/ml and 400 mg/ml respectively. The minimum bactericidal concentration (MBC) for Moringa and Ginger was 400 mg/ml and 800 mg/ml respectively. The docking results revealed that compounds Shogasulfonic C, 5-Hydrohexahydrocurcumin from ginger, Vicenin, Quercetin-3-o-malonylglucoside, Nicotiflorin, Chlorogenic acid, Rutin from moringa have the highest binding affinity with Dihydropteroate synthase, DNA gyrase B, FIMH adhesin, Par E subunit DNA topoisomerase IV and Enoyl acyl carrier protein reductase. The result of the in-vitro assay with the molecular docking studies revealed Moringa leaves and Ginger rhizome antibacterial activity and potential to kill E. coli by inhibiting the proteins, dihydropteroate synthase, DNA gyrase B, FIMH adhesin, Par E subunit DNA topoisomerase IV, and Enoyl ACP reductase.
Conclusion: The discovery of these compounds has highlighted the importance for drug design and development.
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