Novel kinetic spectrophotometric determination of Artesunate using iodide/iodate mixture
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Résumé
Background: Plant materials has been in use globally for medical reasons with little or no knowledge of
phytochemicals therein present and often times no scientific basis.
Methods: A simple kinetic spectrophotometric determination of artesunate is develop. Four kinetic methods
were evaluated, the initial rate method, the fixed time method, variable time method and the rate constant
method. This kinetic spectrophotometric method was based on the redox reaction of artesunate with the
iodate/iodide mixture; in acid condition hydrogen peroxide is generated in situ from the artesunate which then
reacts with iodate/iodide mixture.
Results: The whole four kinetic methods tested all obeyed Beer’s law and were quantitative. The initial rate and
the fixed time methods were most sensitive. The calibration curve generated through the least square method
were linear and the range was 20 – 70 ?g/ml and 10 - 70?g/ml with correlation Coefficient of 0.9998 and
0.9997 respectively, the sensitivity of both kinetic methods were tested as per the ICH guidelines the limit of
detection was 0.022µg/ml, 0.182?g/ml respectively. The accuracy and precision of both kinetic methods was
determined as R.E% and RSD% which in all cases were < 2.8%. The methods were statistically tested by
comparing them with values from a pharmacopoeia standard via students T and variance ratio test (t and F)
Conclusion: The applicability of the methods was ascertained by standard addition method and was find that
pharmaceutical excipients have no effect on the overall result of the developed methods.
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