Amelioration of Isoniazid induced Oxidative Stress and Hematotoxicity by Vitamin C in Wistar Rats
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Résumé
Background: This study was carried out to investigate the ameliorative effect of vitamin C against isoniazid-induced toxicity in some hematological and biochemical parameters in Wistar rats.
Methods: A total of seventy-two (72) adult Wistar rats of both sexes were used which were randomly distributed into 6 groups of 12 rats each. Isoniazid (INH) and Vitamin C were orally administered daily for 2 weeks and on the 7th and 14th day of the experiment, 6 rats from each group were humanely euthanized using ethyl ether and blood was collected for determination of antioxidant, and hematological parameters.
Results: This study revealed significant increase in the level of Malondialdehyde (MDA) in rats treated with INH only. These markers were significantly reduced following co-administration with vitamin C (10 mg/kg and 20 mg/kg) respectively. There was also significant reduction in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH) in rats treated with INH only as compared to the control (distilled water) rats. These markers were significantly increased following co-administration with vitamin C (10 mg/kg & 20mg/kg) respectively. The study also showed that there was no significant difference between the test groups and the control group in hematological parameters.
Conclusion: This finding is indicative of hepatoprotective and antioxidant effect of vitamin C.
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