Evaluation Of The Protective Potential Of Methanol Leaf Extract Of Pyrenacantha staudtii Hutch And Dalz (Icacinaceae) And 3-Carbomethoxypyridine Isolated From It On Chronically-Induced Liver Damage In Rats

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V. N. Ogbeide
S. O. Okpo
G. I. Eze

Résumé

Pyrenacantha staudtii Hutch and Dalz (Icacinaceae) is a woody climber used in folk medicine for the treatment of stomach colic, gastric ulcer, dysmenorrhea, threatened abortion and liver diseases. Earlier studies with 3-carbomethoxypyridine (3-CMP) isolated and characterized from P. staudtii had shown potential hepatoprotective effects on acute hepatocellular damage. The present study evaluates the hepatoprotective effects of the methanol leaf extract of P. staudtii (PS) and 3-CMP isolated from it on chronically-induced hepatocellular damage, using rodent models of hepatotoxicity and assesses the short-term toxicity profile of 3-CMP.Rats were administered P. staudtii (25- 100 mg/kg body weight/day) and 3-CMP (5-20 mg/kg body weight/day) orally for 56 days. Liver damage was induced by twice weekly intraperitoneal administrations of carbon tetrachloride (CCl4, 1 ml/kg), or thioacetamide (TAA, 200 mg/kg) for eight weeks. Body weight changes, biochemical {alanine transaminases (ALT), aspartate transaminases (AST), alkaline phosphatase (ALP), albumin and bilirubin} and histopathologic parameters were evaluated. The urine ascorbic acid content of the 3-CMP treated rats was also determined. A 42-day short-term oral toxicity evaluation of 3-CMP was also carried out. Oral administration of the methanol extract, for 56 days, did not significantly decrease the serum enzyme levels or increase the total protein/albumin levels but reversed the decreased body weight induced by CCl4. Treatment with 3-CMP significantly (p<0.05) and dose-dependently increased the total body weight and urine ascorbic acid content and reduced the ALT, AST and ALP levels without affecting bilirubin or total protein in CCl4-intoxicated rats. However, 3-CMP had no significant effects on the body weight changes, biochemical markers or urine ascorbic acid content in TAA-hepatotoxic rats. Liver sections from both CCl4- and TAA-hepatotoxic rats showed improved histological appearances on treatment with all doses of 3-CMP and P. staudtii. 3-CMP possesses significant antihepatotoxic effect greater than methanol extract of PS, as seen by its ability to decrease liver enzymes increased by CCl4, in a dose-dependent manner, similar to the standard drug sylimarin. This was further confirmed by its effect on urine ascorbic acid content as well as improved liver histology. The more pronounced effect of 3-CMP on CCl4-induced than on TAA-induced liver damage suggests that it might have greater efficacy in liver fibrosis than cirrhosis. Short term toxicity studies indicated that 3-CMP has no immediate or delayed toxic effects on rats.

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Ogbeide, V. N., Okpo, S. O., & Eze, G. I. (2016). Evaluation Of The Protective Potential Of Methanol Leaf Extract Of Pyrenacantha staudtii Hutch And Dalz (Icacinaceae) And 3-Carbomethoxypyridine Isolated From It On Chronically-Induced Liver Damage In Rats. Nigerian Journal of Pharmaceutical and Applied Science Research, 5(1), 21–35. Consulté à l’adresse http://mail.nijophasr.net/index.php/nijophasr/article/view/108
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V. N. Ogbeide

Department of Pharmacology and Toxicology, University of Benin, P. M. B. 1154, Benin City 300001, Nigeria.

S. O. Okpo

Department of Pharmacology and Toxicology, University of Benin, P. M. B. 1154, Benin City 300001, Nigeria.

G. I. Eze

Department of Anatomy, School of Basic Medical Sciences, University of Benin, Benin City 300001, Nigeria

Références

Aguwa CN, Mittal GC (1978). Study of the anti-ulcer activity of aqueous extract of the leaves of Pyrenacantha staudtii (Icacinaceae) using various models of experimental gastric ulcer in rats. Eur J Pharmacol 74: 215-219.

Aguwa CN, Okunji CO (1986).Gastrointestinal studies of Pyrenacantha staudtii leaf extracts. J Ethnopharmacol 151(1): 45-55.

Akubue PI, Mittal GC, Aguwa CN (1983). Preliminary pharmacological study of some Nigerian medicinal plants. J Ethnopharmacol 8: 53-63.

Alshawsh, MA, Abdulla MA, Ismail S, Amin ZA (2011). Hepatoprotective effects of Orthosiphon stamineus extract on thioacetamide-induced liver cirrhosis in rats. Evid Based Complement Alternat Med. Article ID: 103039.

Anosike CA, Ugwu UB, Nwakanma O (2008). Effect of ethanol extract of Pyrenacantha staudtii leaves on carbon tetrachloride-induced hepatotoxicity in rats. Biokemistri 20(1): 17-22.

Brattin WJ, Glende EA, Recknagel RO (1985). Pathological mechanisms of carbon tetrachloride hepatotoxicity. Free Rad Biol Med 1(1): 27-38.

Brenner DA, Alcorn JM (1990). Therapy for hepatic fibrosis. Sem Liver Dis 10: 75-83.

Busari AD (1976). Flora of Nigeria and their taxonomic importance, Lagos, Nigeria. Forest Guide Publisher; p. 1025.

Dacie JV, Lewis SM (1991). Practical Haematology, 7th Ed. Churchill Livingstone, New York. 50-56.

Falodun A,Usifoh CO (2006). Isolation and characterization of 3-carbomethoxypyridine from the leaves of Pyrenacantha staudtii (Hutch and Dalz). Acta Pol Pharm Drug Res 63: 235-237.

Falodun A, Chaudhry MA, Choudhary IM (2009). (2009). Phytotoxic and chemical investigations of a Nigerian medicinal plant. Res J Phytochem 3: 13-17

Falodun A, Usifoh CO, Nworgu ZAM (2005). Phytochemical and active column fractions of Pyrenacantha staudtii on isolated rat uterus. Pak J Pharm Sci 18(4): 31-33.

Falodun A, UsifohCO, Nworgu ZAM (2006). Isolation and characterization of 3-carbomethoxypyridine from Pyrenacantha staudtii (Hutch) leaf on isolated rat uterus. Afr J Biotech 5(12): 1271-1276.

Ghai CL (1995). A Textbook of Practical Physiology. Jaypee, India. 119.

Hutchinson J, Dalziel JM (1966). Flora of West Tropical Africa. Crown Agents for Overseas Governments and Administration, London 1: 72

Ishak KG (1982). The liver. In: Riddell RH, ed. Pathology of drug-induced and toxic diseases. New York: Churchill Livingstone, 457–513.

Jendrassik L, Grof P (1938). Quantitative determination of total and direct bilirubin in serum and plasma. Biochemistry 297: 81-89.

John MB (1972). Laboratory Medicine Haematology, 4th Ed, CV Mosby Co, St. Louis, p. 1198.

Kamble MB, Dumbre RK, Rangari VD (2008). Hepatoprotective activity studies of herbal formulations. Int J Green Pharmacol 2(3): 147-151.

Kim KH, Bae JH, Cha SW, Han SS, Park KH, Jeong TC (2000). Role of metabolic activation by cytochrome P-450 in thioacetamide-induced suppression of antibody response in male BALB/c mice. Toxicol Lett 14(1-3): 225-235.

Leopold CS, Lippold BC (1995). Enhancing effects of lipophillic vehicles on skin penetration of methyl nicotinate in vivo. Pharm Sci 84(2): 195-198

Mesia GK, Tona GL, Penge O, Lusakibanza M, Nanga TM, Cimanga RK, Apers S, Van Miert S, Totte J, Pieters L, Vlietinck AJ (2005). Antimalarial activities and toxicities of three plants used as traditional remedies for malaria in the Democratic Republic of Congo: Croton mubango, Nauclea pobeguinii and Pyrenacantha staudtii. Ann Trop Med Parasitol 99(4): 345-357.

Meyer SA, Kulkarni AP (2001). Hepatotoxicity. In: Hodgson E, Smart, R. C. (eds). Introduction to Biochemical Toxicology, 3rd Ed. John Wiley and Sons Inc., New York, pp 487-490

Mohan GK, Pallavi E, Ravi Kumar B, Ramesh M,Venkatesh S (2007). Activity of carica leaf extract against carbon tetrachloride-induced hepatotoxicity in rats. DARU J PharmSci 15: 162.

Niro HV, Shah W (1986). Vitamins. In: Tietz NW (ed.) Fundamentals of Clinical Chemistry. 2nd edition. WB. Saunders, Philadelphia, pp. 547-550.

OECD (2000) Guidance Document on Acute Oral Toxicity. Environmental Health and Safety Monograph Series on Testing and Assessment, No. 24.

Okpo SO, Falodun A, Inegbenebor ME, Eze GI (2013). Hepatoprotective effect of 3-carbomethoxypyridine isolated from Pyrenacantha staudtii (Icacinaceae) leaves on carbon tetrachloride-induced liver damage. Nig J Pharmaceut Sci 12(1): 55-65

Okpo SO, Orhue J, Falodun A, Alonge PO, Airemwen CO (2012). Evaluation of the gastro-protective activity of 3-carbomethoxypyridine isolated and characterized from the leaves of Pyrenacantha staudtii (Icacinaceae) in rats. J Pharm Biores 9(2): 85-91.

OzerJ, Ratner M, Shaw M, Bailey W, Schomaker S (2008). The current state of serum biomarkers of hepatotoxicity. Toxicology 245: 194-205.

Ray KW, Flamm SL, Di Bisceglie AM, Bodenheimer HC (2008). Serum activity of alanine aminotransferase (ALT) as an indicator of health and disease. Wiley InterSci 47: 1365-1370.

Reddy BP, Murthy VM, Venkateshwarlu V, Kokate CK, Rambhau D (1992). Antihepatotoxic activity of Phyllantus amarus, Tinospora cordifolia, and Ricinus communis. Indian Drugs 30: 338-341.

Reitman S, Frankel S (1957). A colourimetric method for the determination of serum glutamic oxaloacetic acid and serum glutamic pyruvic transaminases. Am J Clin Pathol 28:56

Rikans LE, Hornbrook KR,Cai Y (1994). Carbon tetrachloride hepatotoxicity as a function of age in female Fischer 344 rats. Mech Ageing Dev 76: 89-99.

Roe JH, Kuether CA (1943). The determination of ascorbic acid in whole blood and urine through the 2, 4-dinitrophenyhydrazine. J BiolChem 147: 399-407.

Sadasivan S, Latha PG, Sasikumar JM, Rajashekaran S, Shyamal S, Shine VJ (2006). Hepatoprotective studies on Hedyotis corymbosa (L.) Lam. J Ethnopharmacol 106(2): 245-249.

Saraswat B, Visen P, Dayal R, Agarwal DP, Patnaik GK (1996). Protective action of ursolic acid against chemical induced hepatotoxicity in rats. Indian J Pharmacol 28: 232-239.

Teo S, Stirling D, Thomas S, Hobermann A, Kiorpes A, Khetani V (2002). A 90-day oral gavage toxicity study of D-methylphenidate and DL-methylphenidate in Sprague-Dawley rats. Toxicology 179: 183-187.

Thomas L (1998). Clinical Laboratory Diagnostics, 1st Edition, Frankfurt, TH-books, Verlagsegeselleschaft, pp. 644 - 647

Visweswaram D, Rao PR, Satyanarayana S(1994). A non-invasive method for evaluating hepatoprotective drugs against carbon tetrachloride induced hepatotoxicity. Indian J Pharmacol 26 (4): 301-303.

Winder CV, Lembke LA, Stephens MD (1969). Comparative bioassay of drugs in adjuvant induced arthritis in rats, flufenamic acid, mefenamic acid and phenylbutazone. Arth Rheum 12: 472-482.

Young DS, Pestarter LC, Gibberman V (1975). Effect of drugs on clinical laboratory tests. Clin Chem 21(5): 1D-432D.