Evaluation of Antioxidant Activity And Chemical Analysis Of The Leaf Of <em>Telfairia occidentalis</em>
Article Sidebar
Main Article Content
Abstract
The methanol extract of Telfairia occidentalis leaf and n-hexane, ethyl acetate, butanol fractions were evaluated for their free radical scavenging activity, with DPPH assay. n-hexane fraction had the highest activity exhibiting an IC50 of 78.50?g/ml, comparable to that of the commercial antioxidant BHT. Chromatographic analysis showed the presence of three components in n-hexane fraction (2H1, 2H3 and 2H4) with Rf values of 0.86, 0.71 and 0.55, respectively. Two components were seperated in ethyl acetate fraction (2E1 and 2E5) with Rf values of 0.85 and 0.25, respectively, while the three components of butanol fraction (2B1, 2B2, and 2B3) have Rf values of 0.90, 0.88 and 0.83 respectively. In addition, total phenolic content of T. occidentialis was determined as catechin equivalents. The n-hexane fraction which had the highest DPPH free radical scavenging activity also had the highest total flavonoid contents. The high flavonoid content was responsible for the antioxidant and free radical scavenging activities of Telfairia occidentalis leaf.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Ames, B. M.(1983). Dietary Carcinogens and Anticarcinogens: Oxygen Radical and Degenerative Diseases. Science, 221:1256-
Aruoma, O. I. (1998). Free Radicals, Oxidative Stress and Antioxidants in Human Health. J. Am. Oil Chem. Soc., 75:199-212.
Adedapo, A. A., Jimoh, F. O., Koduru, S., Afolayan, A. J. and Masika, P. J. (2008). Antibacterial and Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Calpurnia aurea. Lincensed Biomed Central Ltd.
Brand-Williams, W., Givelier, M. E. and Berset, C. (1995). Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm Wiss Technol., 28:25-30.
Bondet, V., Brad-william, W. and Berset, C. (1997). Kinetics and Mechanism of Anti-Oxidant Activity Using DPPH Free Radical
Method. Lebensm. Wiss Technol., 31:609-615. Behera, B. C., Verma, N., Sonone, A. and Makhija, U. (2006). Determination of Antioxidative Potential of Lichen Usnea ghattensis in Vitro., LWT, 39:80-85.
Aderibigbe, A.O., Lawal, B. A. S . and Oluwagbemi, J. O. (1999). The Antihyperglycaemic effect of Telfairia occidentalis in Mice. Afr. J. Med Sci., 28: 171- 175.
Eseyin, O. A., Oforah, E. and Dooka, B. D. (2000). Preliminary Study of the Hypoglycemic Action of the Extract of Leaf of Telfairia occidentalis in Normoglycaemic Guinea Pigs. Glob. J. Pure Applied Sci., 6:639-641.
Eseyin, O. A., Igboasoiyi, A. C., Mbagwu, H., Umoh, E. and Ekpe, J. F. (2005). Studies of the Effects of Alcohol Extract of Leaf of Telfairia
occidentalis on Alloxan-induced Diabetic Rats. Global J. of Pure and Applied Science, 2(1):85-87.
Eseyin, O. A., Igboasoiyi, Oforah, E., Nkop, N. and Agboke, A. (2005). Hypoglycemic Activity of Telfairia occidentalis in Rats. J. Pharm.
Bioresource, 2:36-42.
Eseyin, O. A., Ebong, P., Ekpo, A., Igboasoiyi, A. C. and Oforah, E. (2007). Hypoglycemic Effects of the Seed Extract of Telfairia occidentalis in Rat. Pakistan J. of Biol. Sci., 10(3):498-501.
Oboh, G., Nwanna, E. E. and Elusiyan, C. A. (2006). Antioxidant and Antimicrobial Properties of Telfairia occidentalis (Fluted Pumpkin) Leaf Extracts. J. of Pharmacology and Toxicology, 1(2): 167-175.
Oboh, G. and Akindahunsi, A. A. (2004). Change in the Ascorbic Acid, Totol Phenol and Antioxidant Activity of Sun-Dried Commonly
Consumed Green Leafy Vegetables in Nigeria. Nutr. Health, 18:29-36.
Feher, J., Cosmos, G. and Vereckei, A. (1987). Free Radical Reactions in Medicine, Berlin – Heidelberg: Springer-Verlag, pp. 40-43.
Geber, M., Boutron-Ruault, M. C., Hereberg,
S., Riboli, E., Scalbert A. and Siess, M. H. (2002). Food and Cancer: State of the Art about the Protective Effect of Fruits and
Vegetables, 89:293-312.
Johnson, I. T. (2001). Antioxidants and Antitumour Properties. In: Antioxidants in Food, Pokorny, J., Yanishlieva, N. and Gordon,
M. (Eds.) Cambridge: Woodhead Publishing Ltd., pp. 100-123.
Kayode, O. T., Kayode, A. A and Adetola, A. A., (2009). Therapeutic Effect of Telfairia occidentalis on Protein Energy Malnutrition-
Induced Liver Damage. Res. J. Med. Plant, 3: 80- 92.
Kayode, A. A. A., Kayode, O. T. and Adetola, A. A. (2010). Telfairia occidentalis Ameliorates Oxidative Brain Damage in Malnourished Rat. Int. J. Biol. Chem., 4:10-18.
Iweala, E. E. J. and Obidoa, O. (2009). Some Biochemical, Haematological and Histological Responses to a Long Term Consumption of
Telfairia occidentalis- Supplemented Diet in Rats. Pak. J. Nutr., 8:1199 – 1203.
Kayode, A. A. A and Kayode, O. T., (2011). Some Medicinal Values of Telfairia occidentalis: A Review. Am. J. Biochem. Mol. Biol., 1:30-38.
Marinova, D., Ribarova, F. and Atanasova, M. (2005). Total Flavonoid Content Assay Method. J. Univ. Chem. Tech. Metall., 40(3):255.
Miller, A. L. (1996). Antioxidant Flavonoids: Structure, Function and Clinical Usage. Alt. Med. Rev., 1:103-111.
Pietta, P. (2000). Flavonoids as Antioxidants. J. Nat. Prod., 63:1035-1042.
Singleton, V. L. and Rossi, J. A. Jr. (1965). Method for Determination of Total Phenol Content. Am. J. Enol. Viticut., 16:144.
Shahidi, F. and Wanasundara, P. K. J. P. D. (1992). Phenolic Antioxidant. Crit. Rev. Food Sci. Nutri., 32:67-103.
Virgili, F., Scaccini, C., Packer, L. and Rimbach, G. (2001). Cardiovascular Disease and Nutritional Phenolics. In: Antioxidants in
Food, Pokorny, Yanishlieva, J. N. and Rimbach, G. (Eds.). Cambridge: Woodhead Publishing Ltd., pp. 87-99.
Wilson, R. L. (1988). Free Radicals and Tissue Damage, Mechanistic Evidence from Radiation Studies. In Biochemical Mechanism
of Liver Injury. New York: Academy Press, p. 123.
Wei, Z. and Shiow, Y. W. (2001). Antioxidant Activity and Phenol Compounds in Selected Herbs. J. Agric. Food Chem., 49:5165-5170.
Pratt, D. E. (1992). Natural Antioxidants from Plant Material. Sandiego: C. T. Press Inc., p. 424.
Yang, J. H., Lin, H.C and Mau, J. L. (2002). Antioxidant Properties of Several Commercial Mushrooms. Food Chem., 77:229-235.