Phytochemical Evaluation and Combination Effect of the Crude Methanol Extract of Ochna rhizomatosa with Standard drugs

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J. D. Habila
M. M. Adeyemi
O. R. A. Iyun
S. E. Paul
A. J. Habila
M. I. Mohammed

Abstract

In our search for plants with antimicrobial activities, Ochna rhizomatosa a plant used in the treatment of typhoid fever in Nigerian folk medicine was investigated. The phytochemical investigation of the extract shows the presence of alkaloids, flavonoids, fatty acid and triterpenes. The results of antimicrobial assay on Samonella typhi, two resistant bacteria (Methicillin Resistance Staphyloccocus aureous (MRSA); Vancomycin Resistance Staphyloccocus aureous (VRSA)) and Candida albican expressed in terms of mean diameter of zones of inhibition (mm), shows that the leaves extract (LOS1) inhibited MRSA with a clear zone of 28.00 ± 0.20 mm. Whereas the stem bark (SOS1) shows a zone of 18.00 ± 0.50 mm at 100 mg/ml respectively. The combination of LOS1(100 mg/ml) with Ciprofloxacin (5 ?g/ml), led to enhancement of the measured zone of inhibition (46.00± 0.25 mm). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) results of the synergistic evaluation of LOS1 with Ciprofloxacin, reveals an enhancement of the inhibition (MRSA, MIC = 3.13 mg/ml) and bactericidal properties (MRSA, MIC = 3.13 mg/ml, MBC = 6.25 mg/ml). These showed that the standard drugs in combination with the extract from Ochna rhimatoza are potentials for the search for treatment for resistant bacteria.

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Habila , J. D., Adeyemi, M. M. ., Iyun, O. R. A. ., Paul , S. E., Habila , A. J., & Mohammed , M. I. (2020). Phytochemical Evaluation and Combination Effect of the Crude Methanol Extract of Ochna rhizomatosa with Standard drugs. Nigerian Journal of Pharmaceutical and Applied Science Research, 8(1), 52–55. Retrieved from http://mail.nijophasr.net/index.php/nijophasr/article/view/278
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Author Biographies

J. D. Habila

Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.

M. M. Adeyemi

Department of Chemistry, Nigeria Defense Academic, Kaduna, Nigeria.

O. R. A. Iyun

Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.

S. E. Paul

Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria

A. J. Habila

Department of Laboratory Science Technology, Nigeria Institute of Leather Science and Chemical Technology (NILEST), Samaru-Zaria, Nigeria.

M. I. Mohammed

Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria.

References

Cosa, P, Vlietinck, AJ, Berghe, DV, Maes, L 2006, ‘Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’ Journal Ethnopharmacology. Vol.106, pp. 290

Duraipandiyan, V, Ayyanar, M & Ignacimuthu, S 2006, ‘Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India’. BMC Complementary Alternative Medicine. Vol. 6, no. 35, pp. 41.

Nascimento, GGF, Locatelli, J, Freitas, PC & Silva GL, 2000, ‘Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria’. Brazilian Journal of Microbiology. Vol. 31, no.1, pp. 247-256.

Abu-Shanab, B, Adwan, G, Abu-Safiya, D, Adwan, G & Abu-Shanab, M 2005, ‘Antibacterial activity of Rhus coriaria. L extracts growing in Palestine’. Journal of Islamic University Gaza, Natural Science Series. Vol. 13, no. 1, pp. 147-153.

Adwan, MG, Abu-shanab AB & Adwan, MK 2008, ‘Invite activity of certain drugs in combination with plants extracts against staphylococcus aureus infections’ Pakistan Journal of Medicine. Vol. 24no.1 pp.541-544.

Chang, PC, Li, HJ,Tang, HJ, Liu, JJ, Wang, JW & Chuang, YC 2007, ‘Invitro synergy of baicalein and gentamicin against vancomycin-resistant enterococcus’, Journal of Microbiology & Immunology Infections.Vol. 40, no.1, pp.56-61.

Horiuchi, K, Shiota, S, Kuroda, T, Hatano, T, Yoshida, T & Tsuchiya, T 2007, ‘Potentiation of antimicrobial activity of aminoglycosides by carnosol from Salvia officinalis’. Biology Pharmaceutical Bulletin. Vol. 30, no.1, pp. 287-290.

Burkill, HM 1985, ‘The useful plants of West Tropical African’, 2nd edn Vol. 4. McGraw-Hill, New York.

Oliveria, MCC, Carvalho, MG, Silva, CJ & Werle, AA, 2002, ‘New bioflavonoid and other constituents from Luxemburgianobilis eichl’. Journal of Brazilian Chemical Society. Vol. 13, no.1, pp.119-123.

Sofowora, A 2008, ‘Phytochemical screening. Medicinal Plants and Traditional Medicine in Africa’ 3rd edn, Spectrum books limited, Ibadan, Nigeria. pp. 199-204.

Evans, WC& Trease T, 1996,‘Pharmacognosy’. 14th edn. London: WB Saunders Ltd, pp. 119–159.

Bauer AW & Kirby, WMM, 1996, American Journal of Clinical Pathology, Vol. 45, no. 1, pp.493.