Novel kinetic spectrophotometric determination of Artesunate using iodide/iodate mixture

Main Article Content

Emmanuel E. Attih
Wale H. Oladimeji
Olorunfemi A. Eseyin
Victor E. Attih

Abstract

Background: Plant materials has been in use globally for medical reasons with little or no knowledge of


phytochemicals therein present and often times no scientific basis.


Methods: A simple kinetic spectrophotometric determination of artesunate is develop. Four kinetic methods


were evaluated, the initial rate method, the fixed time method, variable time method and the rate constant


method. This kinetic spectrophotometric method was based on the redox reaction of artesunate with the


iodate/iodide mixture; in acid condition hydrogen peroxide is generated in situ from the artesunate which then


reacts with iodate/iodide mixture.


Results: The whole four kinetic methods tested all obeyed Beer’s law and were quantitative. The initial rate and


the fixed time methods were most sensitive. The calibration curve generated through the least square method


were linear and the range was 20 – 70 ?g/ml and 10 - 70?g/ml with correlation Coefficient of 0.9998 and


0.9997 respectively, the sensitivity of both kinetic methods were tested as per the ICH guidelines the limit of


detection was 0.022µg/ml, 0.182?g/ml respectively. The accuracy and precision of both kinetic methods was


determined as R.E% and RSD% which in all cases were < 2.8%. The methods were statistically tested by


comparing them with values from a pharmacopoeia standard via students T and variance ratio test (t and F)


Conclusion: The applicability of the methods was ascertained by standard addition method and was find that


pharmaceutical excipients have no effect on the overall result of the developed methods.

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How to Cite
E. Attih, E., Oladimeji, W. H., Eseyin , O. A., & Attih , V. E. (2023). Novel kinetic spectrophotometric determination of Artesunate using iodide/iodate mixture. Nigerian Journal of Pharmaceutical and Applied Science Research, 10(3), 59–67. Retrieved from http://mail.nijophasr.net/index.php/nijophasr/article/view/516
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