A Antimicrobial Activity of Citrus aurantifolia Peel Essential Oil Against Clinically Relevant Bacteria and Fungi
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Résumé
Abstract
Background: The increasing tolerance of several microorganisms against commonly used antibiotics represents a
challenge for microbiologists to find alternative ways for the treatment of such infections. A study was carried out
to determine the effect of Citrus aurantifolia oil extract on clinical bacteria and fungi obtained from routine laboratory
benches of the department of pharmaceutical microbiology, University of Ibadan.
Methods: Citrus aurantifolia peels oils were extracted by hydro-distillation using a Clevenger-type apparatus,
distillates of essential oils were dried over anhydrous sodium sulfate, filtered and concentrated at 30oC using a rotary
evaporator. Conventional biochemical characterization of the isolates and antibacterial assay of the extract on the
isolates was carried out.
Results: The antibacterial activities of the essential oil from Citrus aurantifolia peels on test isolates tested exhibited
zones of inhibition that ranged between 15mm and 20mm. The minimum inhibitory concentration, minimum
bactericidal concentration and minimum fungicidal concentration showed antimicrobial and anti-fungicidal activity
on all tested isolates, with MIC values range between 0.39 mg/mL as obtained for Salmonella typhi and Bacillus
subtilis and 3.125mg/mL as recorded in Staphylococcus aureus and Klebsiella pneumonia while the MFC values
that range between 0.391 and 0.78 as were recorded in Trichophyton rubrum and Candida albicans.
Conclusion: Essential oils of Citrus aurantifolia peels demonstrated remarkable antibacterial and anti-fungicidal
activity on all the isolates tested, which is suggestive of its antimicrobial potential and may be used as therapeutic
agent for the treatment of microbial infections when further purified.
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