Kinetics and Mechanisms of Doxorubicin Release from hydroxyapatite-sodium alginate nanocomposite
Article Sidebar
Main Article Content
Abstract
In-vitro kinetics and mechanistic study helps the formulation and research scientists to forecast possible rate and mechanism of in-vivo drug release. This study investigates the kinetics and mechanism of Doxorubicin (DOX) (an anticancer drug) release from hydroxyapatite-sodium alginate nanocomposites (HASA). In-situ preparation of hydroxyapatite (HA) in the presence of sodium alginate (SA) was done by wet chemical precipitation method. Drug loading was carried out at neutral pH, while in vitro drug release study was carried out in synthetic body fluid (SBF) at pH 7.4 and 37 0C. The release experimental data was fitted into model-dependent and model independent methods using DDSolver software, an excel add-in. The release half time (t50) increased with increase in SA content. Except for HA and HASA-1%wt, the release of DOX from other formulations can best be explained by first order kinetics. The value of n exponent in Korsmeyer-Peppas model ranged from 0.220 to 0.497, which indicate that DOX release from all the formulations followed Fickian diffusion mechanism. The results of profile comparison indicate that the following release profiles are similar: HASA-5%wt and HASA-20%wt, HASA-20%wt and HASA-33%wt, HASA-33%wt and HASA-50%wt. Addition of SA to HA prolonged the release of DOX and also influenced the kinetics and the mechanism of DOX release from the nanocomposite.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Adams, E., Coomans, D., Smeyers-Verbeke, J and Massart, D.L. (2001). Application of linear mixed effects models to the evaluation of dissolution profiles. International Journal of Pharmarcy, 226, 107-25.
Badri, W., Eddabra, R., Fessi, H and Elaissari, A. (2014). Biodegradable Polymer Based Nanoparticles: Dermal and Transdermal Drug Delivery. Journal of Colloid Science and Biotechnology, 3, 141 - 149.
Biondi, M., Ungaro, F., Quaglia, F and Netti, P.A. (2008). Controlled drug delivery in tissue engineering. Advances in Drug Delivery Review, 60, 229-227.
Bonacucina, G., Cespi, M and Palmieri, G.F. (2009). Evaluation of dissolution kinetics of hydrophilic polymers by use of acoustic spectroscopy. International Journal of Pharmacy, 377, 153-158.
Chime, S.A., Onunkwo, G.C and Onyishi, I. (2013). Kinetics and Mechanisms of Drug Release from Swellable and Non Swellable Matrices: A Review. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 4(2), 97-103.
Cojocariu, A., Porfire, L., Cheaburu, C and Vasile, C. (2012). Chitosan/montmorillonite nanocomposites as matrices for prolonged delivery of some novel nitric oxide donor compounds based on theophylline and paracetamol. Cellulose Chemistry Technology, 46 (1-2), 35-43.
Comyn, J. (1985). Introduction to Polymer Permeability and the Mathematics of Diffusion, In: Polymer Permeability, pp 1-10, Elsevier Applied Science, ISBN 0-85334-322-5, Northern Ireland.
Dash, S., Murthy, P.N., Nath, L and Chowdhury, P. (2010). Kinetic modeling on drug release from controlled drug delivery systems. Acta Poloniae Pharmaceutica – Drug Research, 67(3), 217-223.
Ford, J.L, Rubinstein, M.H and Hogan, J.E (1985). Propranolol hydrochloride and aminophylline release from matrix tablets containing hydroxypropyl methylcellulose. International Journal of Pharmacy, 24, 339-350.
Higuchi, T. (1961). Rate of release of medicaments from ointment bases containing drugs in suspension. Journal of Pharmaceutical Science, 50, 847-875.
Huang, X and Brazel, C.S. (2001). On the importance and mechanisms of burst release in matrix-controlled drug delivery systems. Journal of Controlled Release, 73, 121-136.
Jesus, R.A., Rabelo, A.S., Figueiredo, R.T., Cides da Silva L.C., Codentino, I.C., Fantini, M.C.A., G.L.B. Araújo, G.L.B., Araújo, A.A.S and Mesquita, M.E. (2016). Synthesis and application of the MCM-41 and SBA-15 as matrices for in vitro efavirenz release study. Journal of Drug Delivery Science and Technology, 31, 153-159. http://dx.doi.org/10.1016/j.jddst.2015.11.008
Karimi, M. (2011). Diffusion in Polymer Solids and Solutions, Mass Transfer in Chemical Engineering Processes, Dr. Jozef Marko Å (Ed.), ISBN: 978-953-307-619-5, InTech.
Kanjickal, D.G., and Lopina, S.T (2004). Modeling of drug release from polymeric delivery systems-A review. Critical Review Therapeutic Drug Carrier System, 21, 345-386.
Landgraf, W., Li, N.H and Benson, J.R. (2005). New polymer enables near zero order release of drugs. Drug Delivery Technology, 5, 48-55.
Moore, J. W and Flanner, H. H. (1996). Mathematical comparison of dissolution profiles. Pharmaceutical Technology, 20(6), 64-74.
Noyes, A.A and Whitney, W.R. (1987). The rate of solution of solid substances in their own solutions. Journal American Chemical Society, 19, 930-934.
Onoyima C. C, Okibe F. G, and Agbaji E. B. (2017). Preparation and in vitro evaluation ofhydroxyapatite-sodium alginate nanocomposite for sustained delivery of doxorubicin.African Journal of Pharmaceutical Research and Development, 9(2), 154-16.
Peppas, N.A and Korsmeyer, R.W. (1986). Dynamically swelling hydro gels in controlled release applications, in: N.A. Peppas (Ed.), Hydrogels in Medicine and Pharmacy, Vol. 3, CRC Press, Boca Raton, pp. 109-136.
Raval, A., Parikh, J and Engineer, C. (2010). Mechanism of Controlled Release Kinetics fromMedical Devices. Brazilian Journal of Chemical Engineering, 27( 02), 211-225.
Shaikh, K.H., Kshirsagar, R.V and Patil, S.G. (2015). Mathematical models for drug release characterization: A review. World Journal of Pharmacy and Pharmaceutical Sciences, 4(4), 324-338.
Shende, M.A and Marathe, R.P. (2015). Formulation and development of baclofen gastro retentive mucoadhesive tablets using hibiscus esculentus and xanthan gum. Indo American Journal of Pharmaceutical Research, 5(9), 2925 – 2938.
Siegel, R.A and Rathbone, M.J. (2012). Overview of controlled release mechanism, in Fundamentals and Applications of Controlled Release Drug Delivery, Advances in Delivery Science and Technology, J. Siepmann et al. (eds.), Controlled Release Society. DOI 10.1007/978-1-4614-0881-9_2.
Siepmanna, J and Peppas, N.A. (2001). Modelling of drug release from delivery systems based hydroxypropyl methylcellulose (HPMC). Advanced Drug Delivery Reviews, 48, 139-157
Singhvi, G and Singh, M. (2011). Review: in-vitro drug release characterization models. International Journal of Pharmaceutical Studies and Research, 2(1), 77-84.
Zhang, Y., Huo, M., Zhou, J., Zou, A., Li, W., Yao, J and Xie, X. (2010). DDSolver: An Add-In Program for Modeling and Comparison of Drug Dissolution Profiles. American Association of Pharmaceutical Scientists Journal, 12(3), 263-271. DOI: 10.1208/s12248-010-9185-1.
Zuo, J., Gao, Y., Bou-Chacra, N and Löbenberg, R. (2014). Evaluation of DDsolver software application. BioMed Research Internationl, http://dx.doi.org/10.1155/2014/204925