Comparative Assessment of the Nutritional and Antioxidant Status Of Euphorbia Heterophylla (Euphorbiaceae) And Morinda Lucida (Rubiaceae) Plants

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Evuen Uduenevwo Francis
Apiamu Augustine
Owuzo Patience Ozioma

Abstract

This study comparatively and quantitatively ascertained the antioxidant, nutritional and phytochemical potentials of Euphorbia heterophylla and Morinda lucida plants by Standard methods. The results of moisture, crude protein, fibre and ash contents respectively obtained in proximate analysis were significantly higher (p<0.05) in Euphorbia heterophylla than that of Morinda lucida except that of the total fat and carbohydrate contents. In mineral analysis, the relative amounts of potassium, calcium, magnesium, zinc and iron in the leaves of Morinda lucida were significantly higher (p<0.05) as compared to E. heterophylla. With phytochemical analysis, there was a high significant difference (p<0.05) of tannins, alkaloids and cyanogenic glycosides in both plants, and a higher significant difference (p<0.05) in the amounts of phytate, oxalate and phenols in Morinda lucida than that of Euphorbia heterophylla. There was also, a significant difference (p<0.05) for the antioxidant capacity of both plants with predominance of vitamin C, catalase, and superoxide dismutase activities in Euphorbia heterophylla than that of Morinda lucida. However, Glutathione peroxidase activity predominates in the latter plant. Therefore, these plants are rich in nutrients, phytochemicals and highly valuable sources of natural antioxidants, which may combat oxidative stress and inhibit degenerative disorders.

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Francis, E. U. ., Augustine, A. ., & Ozioma, O. P. . (2020). Comparative Assessment of the Nutritional and Antioxidant Status Of Euphorbia Heterophylla (Euphorbiaceae) And Morinda Lucida (Rubiaceae) Plants. Nigerian Journal of Pharmaceutical and Applied Science Research, 5(2), 49–57. Retrieved from http://mail.nijophasr.net/index.php/nijophasr/article/view/126
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Author Biographies

Evuen Uduenevwo Francis

Department of Biochemistry, College of Natural and Applied Sciences, Western Delta University, P.M.B. 10, Oghara, Delta State, Nigeria.

Apiamu Augustine

Department of Biochemistry, College of Natural and Applied Sciences, Western Delta University, P.M.B. 10, Oghara, Delta State, Nigeria.

Owuzo Patience Ozioma

Department of Biochemistry, College of Natural and Applied Sciences, Western Delta University, P.M.B. 10, Oghara, Delta State, Nigeria

References

Abbasi, M.A., Hina Saleem, H., Rehman, A., Riaz, T. and Ajaib, M. (2013). Determination of Antioxidant Activity and Phytoconstituent Screening of Euphorbia heterophylla Linn.British Journal of Pharmaceutical Research, 3(2): 202-216

Adeneye, A.A. and Agbaje, E.O. (2008). Pharmacological evaluation of oral hypoglycemic and Antidiabetic effects of fresh leaves ethanol extract of Morinda lucida benth in normal and alloxan-induced diabetic rats. African Journal Biomedical Research 11:65-71.

Adesogan, E.K. (1973). Anthraquinones and anthraquinols from Morinda lucida. The biogenic significance of Oruwal and Oruwalol. Tetrahedron; 29:4099-102.

Adnan, M. and Holscher, D. (2010). Medicinal Plant Abundance on Degrade and Reforested Site in Northwest Pakistan. Mountain Research and Development, 30(1), 25-32.

Ajaiyeoba, E.O., Abiodun, O.O., Falade, M.O., Ogbole, N.O., Ashidi, J.S, Happi, C., Akinboro, A., and Bakare, A.A. (2006). Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. Journal Ethnopharmacology, 112, 470-5.

Akinboro A. and Bakare A.A. (2007) Cytotoxic and genotoxic effects of aqueous extracts of five medicinal plants on Allium cepa Linn. Journal of Ethnopharmacology 112: 470-475

Akinmoladun, A.C., Obuoto, E.M. and Farombi, E.O. (2010). Evaluation of antioxidant and free radical scavenging capacities of some Nigerian Indigenous plants. Journal of Medicinal Food :13: 444-51.

AOAC. (2000). Official Methods of Analysis International. 17th edition. Association of Official Analytical Chemists, Washington DC. USA.

AOAC. (1980). Official Methods of Analysis International. 17th edition. Association of Official Analytical Chemists, Washington DC. 376 - 384

AOCS (American Oil Chemist Society) (2000). Official methods of analysis 5th edition. Association of Official analysis chemists Washington, DC, USA.

Arslan, D. and Özcan, M.M. (2008). Evaluation of drying methods with respect to drying kinetics, mineral content and colour characteristics of rosemary leaves. Energy Conversion and Management 49: 1258-1264.

Asuzu, I.U. and Chineme, C.N. (1990). Effects of Morinda lucida leaf extract on Trypanosoma brucei infection in mice. Journal of Ethnopharmacology; 30:307-13.

Ajayeoba, F.O., Abiodun, O.O., Falade, M.O., Ogbole, N.O., Ashidi, J.S., Happi, C.T., and Akinboye, D. O. (2006). In vitro cytotoxicity studies of twenty plants used in Nigerian antimalarial ethnomedicine. Phytomedicine 13(4): 295-298.

Bajai, A.M. (2001).‘’Effect of Natural extract of pineapple on disstibility, performance traits and nitrogen balance of broiler chicks’’. Australian Journal of Basic and applied Sciences, 5(20): 10-30.

Balogun, E.A. and Akinloye, D.I. (2012). Biochemical Effects of Methanolic Extract of Morinda morindoides and Morinda lucida Leaves on Lipid Profile, Bilirubin and Some Marker Enzymes. Asian Journal of Medical Research, 1(1) : 12-16.

Bello, M.O., Falade, O.S., Adewusi, S.R. and Olawole, N.O. (2008). Studies on the chemical compositions and anti-nutrients of some lesser known Nigerian fruits. African Journal of Biotechnology 7 (21): 3972-3979.

Borneo, R., le?n, E.A., Aguirre, A., Ribotta, P., Cantero, J.J. (2008). Antioxidant capacity of medicinal plants from the Province of Cordoba (Argentina) and their in vitro testing in model food system. Food Chemistry. 112, 664-670.

Cheung, L.M., Cheung, P.C.K., Ooi, V.E.C. (2003). Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry: 81:249-255. Claiborne A., (1985).Catalase activity. In: Greenwald R.A., ed. CRC handbook of methods in oxygen radical research. Boca Raton, FL, CRC Press. 283-284.

Coimbra, M.C. and Jorge, N. (2011). Proximate composition of guariroba (Syagrus oleracea), jerivá (Syagrus romanzoffiana) and macaúba (Acrocomia aculeata) palm fruits. Food Research International 44: 2139-2142.

Dokori, O.B. (1998). Herbs of Ghana, (2nd Ed). Ghana University Press, Accra, p766.

Ekpete, O.A. and Edori, O.S. (2013). Proximate and mineral composition of some Nigerian fruits. International Journal of Research and Reviews in Pharmacy and Applied science, 3(2): 287- 295.

Erden, Y. S., Ekrem, H., Gisho, T. and Yoshiohiro, T. (1999). Traditional medicine in Turkey IX, folk medicine in North West Anatolia. Journal of Ethnopharmacology. 64: 201.

Ettar, R.R., and Emeka, P. (2004). Morinda lucida extract induces endothelium dependent and independent relaxation of rat aorta. Fitoterapia 75:332-326.

Falodun A. and Agbakwuru, E.O.P. (2004). Phytochemical Analysis and Laxative Activity of Euphorbia heterophylla Linn (Euphorbiaceae). Pakistan Journal of Scienctific Research. 47(5): 345-348.

Falodun, A. and Agbakwuru, E.O.P, (2004). Phytochemical analysis and Laxatative activity of Euphorbia heterophylla linn (Euphorbiaceae). Pakistan Journal of science Research 47(5): 454-459.

Falodun, A., Agbakwuru, E.O.P. and Ukoh, G.C. (2003). Antibacterial Activity of Euphorbia heterophylla Linn (Family Euphorbiaceae). Pakistan Journal Scienctific Research. 46 (6): 471-472.

Falodun, A., Okunrobo, L. O. and Uzoamaka, N. (2006). Phytochemical screening and anti- inflammatory evaluation of methanolic and aqueous extracts of Euphorbia heterophylla Linn (Euphorbiaceae). African Journal Biotechnology. 6: 529- 531.

Fasola, T.R. and Egunyom,i A. (2005). Nigerian usage of bark in phytomedicine. Ethnobotany Research and Application; 3:73-7.

Goldie U. and Vijay N. (2012). in vitro Antioxidant Activity of Ethanolic Extract of Euphorbia Hirta Linn. International Journal of Current Research 4(8):153-157.

Gough, L.P., Shacklette, H.T. and Case, A.A. (1979). Element concentrations toxic to plants, animals and man. United States Geological Survey Bulletin, 80: 1466.

Hamid A.A, Aiyelaagbe O.O, Usman L.A, Ameen O.M, Lawal A. (2010) Antioxidants: Its medicinal and pharmacological applications. African Journal of Pure and Applied Chemistry 4(8): 142-151.

Haque, M.R. and Bradbury, J.H. (2002). Total cyanide determination of plants and foods using the picrate and acid hydrolysis methods. Food Chemistry; 77(1): 107-114.

Harbone, J.B. (1973). Phytochemical methods, London, Chapman and Hall limited. pp. 49-188.

Hassan, L.G., Usman, B.B., Kamba, A.S. and Hassan, S.W. (2009). Nutritional compositions of vegetable spaghetti. Nigeria Journal of Food science, 5(3):61-68.

Hurrell, R.F. (2003). Influence of vegetable protein sources on trace element and mineral bioavailability. Journal of Nutrition, 3(9): 2973-2977.

Kalpana K, Kodukkur VP (2011). Antihyperglycemic effect of the alcoholic seed extract of Swietenia macrophylla on streptozotocin-diabetic rats. Pharmacognosy Res; 3(1): 67–71.

Katalini?, V., Miloš, M., Kuliši?, T., Juki?, M. (2004). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem. 94, 550-557.

Kuether, C. A. and Roe, J. H. (1941). Proc. Sot. Exp. Biol. and Med., 47,487

Lawal, H.O., Etatuvie, S.O and Fawehinmi. A.B. (2012). Ethnomedicinal and Pharmacological properties of Morinda lucida. Journal of Natural Products, 5:93-99.

Liu, R. (2004), “Potential Synergy of phytochemicals in Cancer prevention. Mechanism of action”, The Journal of Nutrition, 134, 3479-3485.

Lucas, G.M. and Markakas, 1975. Phytic acid and other phosphorus compounds of bean (Phaseolus vulgaris) J. Agric. Edu. Chem., 23: 13-15.

Madziga, H.A., Sanni, S. and Sandabe, U.K. (2010). Phytochemical and Elemental Analysis of Acalypha wilkesiana Leaf. Journal of American Science. 6(11): 510-514.

Mccall, K.A., Huang, C. and Fierke, C.A. (2000). Function and Mechanism of zinc metalloenzymes. Journal of Nutrition, 130: 14375–14465.

Mensor, L.L., S.M. Fabio, G.L. Gildor, S.R. Alexander,C.D. Tereza, Cintia, S.C. and Meskin, M. S. (2001). Phytochemicals in Nutrition and Health. CRC Press. p. 123.

Misra, H. P. and Fridovich, I. (1972). Journal of Biological Chemistry. 247:3170

Muhammad, A. A., Hina, S., Aziz-ur-Rehman, Tauheeda, R. and Muhammad, A. (2013). Determination of Antioxidant Activity and Phytoconstituent Screening of Euphorbia heterophylla Linn. British Journal of Pharmaceutical Research 3(2): 202-216.

Muller, H.G. and Tobin, G. (1980). Nutrition and Food Processing. Croom Helm, London.

Nieves, J.W., Komar, L., Cosman, F. and Lindsay, R. (1998). Calcium potentiates the effect of estrogen and calcitonin on bone mass: review and analysis, American Journal of Clinical Nutrition, 67: 18–24.

Nwoko, E.N. and Bragg, B.B. (2000). Influence of Phytic acid and crude fibre on the availability of minerals from protein supplements in growing chicks. Journal of animal Science, 57:475- 477.

Oduola, T., Bello, I., Adeosun, G., Ademosun, A., Raheem, G. and Avwioro, G. (2010). Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract. North American Journal of Medical Science; 2:230-3.

Ogbonna, A.C., Abuajah, C.I. and Ekpe, G.E. (2013). A comparative study of the nutritive factors and sensory acceptance of juices from selected Nigerian fruits Croatian Journal of Food Technology, Biotechnology and Nutrition 8 (1-2), 47-51.

Okeniyi, S.O., Adedoyin, B. J., and Garba, S.(2012). Phytochemical Screening, Cytotoxicity, Antioxidant and Antimicrobial Activities of Stem and Leave Extracts of Euphorbia heterophylla. Bull. Environ. Pharmacol. Life Sci.; Volume 1 [8]: 87 – 91.

Omale, J. and Emmanuel T.F. (2010). Proximate and nutrient composition of Euphorbia hetrophylla: a medicinal plant from Anyigba, Nigeria. Journal of Medicinal Plants Research 4(14), 1428-1431.

Omale, J., and Emmanuel,T. F. (2010). Phytochemical Composition, Bioactivity and Wound Healing Potential of Euphorbia Heterophylla (Euphorbiaceae) Leaf Extract. International Journal on Pharmaceutical and Biomedical Research 1 (2): 54-63.

Perkins-Veazie, P.M., Collins, J.K. and Robert, W. (2005). Screening carotenoid content in seeded and seedless watermelon fruit. Journal of Horticultural Science 39, 830.

Pugalenthal, M., Vadivel, V., Gurumoorthi, P. and Janardhanam (2004). Comparative nutritional evaluation of little known legumes Tamarandus indica, Erythrina indica, Sesbania bispinosa. Tropical and Subtropical Agroecosystem. 4:107-123.

Raji, Y., Akinsomisoye, O.S. and Salman, T.M. (2005). Antispermatogenic activity of Morinda lucida extract in male rats. Asian Journal of Andrology; 2:405-10.

Ramchoun, M., Harnafi, H., Alem, C., Benlys, M., Elrhaffari, L. and Amrani, S. (2009). Study on antioxidant and hypolipidemic effects of polyphenol rich extract from Thymus vulgaris and Lavendula multifida. Pharmacognosy Research, 1, 106-112.

Reddy, V.K. (2012). Biological scavangers of free radicles -anti-oxidants: A Review. International Journal of Pharmaceutical, Biological and Chemical Sciences, 1(1): 13- 19.

Roe, J.H., and Kuether, C.A. (1943). The determination of ascorbic acid in whole blood and urine through the 2,4- dinitrophenylhydrazine derivative of dehydro-ascorbic acid. J Biol. Chem., 147, 399.

Sanchez-Alonso, F. and M. Lachica (1987). Seasonal trends in the elemental content of plum leaves. Commun. Soil Sci. Plant Anal., 18: 31-44.

Sarker, S.D. and Nahar, L. (2007). Chemistry for Pharmacy Students General, Organic and Natural Product Chemistry. England: John Wiley and Sons. 283-359.

Sen, S., Chakraborty, R., De, B. and Mazumder, J. (2009). Plants and phytochemicals for peptic ulcer: an overview, Pharmacognosy Reviews, 3, 270-279.

Shah, M.T., Begun, S., Khan, S. (2009). Pedo and Biogeochemical Studies of mafic and intramafic rocks in the Mingora and Kabal areas, Swat, Pakistan. Environmental Earth Sciences, 10: 0253 – 0258.

Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299, 152-178.

Sowemimo, A., A., Fakoya, F., A., Awopetu, I., Omobuwajo, O.R., Adesanya, S.A.(2007). Toxicity and mutagenic activity of some selected Nigerian plants. J. Ethnopharmacol.113:427 - 432.

Specker, B.L. (1996). Evidence for an interaction between calcium intake and physical activity on change in bone mineral density. Journal of Bone and Mineral Research, 11: 1539-1544.

Tappel, A.L. (1978). Gluthathione peroxidase and hyperoxides methods. Enzymology. 52:506-513

Tosun, M., Ercisli, S., Sengul, M., Ozer, H., Polat, T. (2009). Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biological Research. 41, 175-181.

Ugwu Okechukwu, P. C., Nwodo Okwesili, F. C., Joshua Parker, E., Bawa Abubakar., Ossai Emmanuel, C. and Odo Christian, E. (2013). Phytochemical and Acute Toxicity Studies of Moringa oleifera Ethanol Leaf Extract. International Journal of Life Sciences Biotechnology and Pharmaceutical Research; 2 (2): 66-71.

Vallee, B.L., Coleman, J.E. and Auld, D.S. (1991). Zinc fingers, zinc clusters and Zinc twist in DNA – binding protein domains. Proceeding of the National. Academy of Sciences., USA; 88: 999-1003.

Van-Burden, T.P., Robinson, W.C. (1981). Formation of complexes between protein and Tannin acid. Journal of Agriculture and Food Chemistry 1: 77.

WHO and FAO (2003). Diet, nutrition & the prevention of chronic diseases: In report of a joint WHO/FAO Expert consultation. Geneva.