@article{Ajala_Makinde_Ogah_2023, title={Inhibition of baker’s yeast alpha glucosidase by extract and fractions of stem bark of Terminalia catappa Linn. (Combretaceae): Terminalia catappa Linn. (Combretaceae) stem bark fractions inhibited baker’s yeast alpha glucosidase}, volume={11}, url={http://mail.nijophasr.net/index.php/nijophasr/article/view/496}, abstractNote={<p style="text-align: justify; line-height: 200%;"><span style="font-size: 14.0pt; line-height: 200%;">Background: Paucity of clinically applicable inhibitors has limited alpha glucosidase inhibition therapeutic strategy of type 2 diabetes management. The current investigation was aimed at evaluating the anti-diabetic alpha glucosidase inhibitory potentials of <em>Terminalia catappa</em> stem bark extract and its fractions, modeling the implicated intestinal brush border membrane alpha glucosidases with the baker’s yeast alpha glucosidase maltase.</span></p> <p style="text-align: justify; line-height: 200%;"><span style="font-size: 14.0pt; line-height: 200%;">Methods: A crude stem bark extract of <em>Terminalia catappa</em> and its n-hexane-, dichloromethane- and methanol-soluble fractions were incubated with the baker’s yeast alpha glucosidase at various final assay mixture concentrations, carrying along acarbose as reference standard.  IC<sub>50</sub> values were calculated from % inhibition vs concentration plots and analyzed using one-way ANOVA with Tukey’s <em>post hoc</em> comparison.</span></p> <p style="text-align: justify; line-height: 200%;"><span style="font-size: 14.0pt; line-height: 200%;">Results<em>: The methanol, n-hexane and dichloromethane fractions inhibited alpha glucosidase with IC<sub>50</sub> values  </em>173.85 ± 6.90 ?g/ml, 258.56 ± 12.90 ?g/ml and 329.90 ± 10.67 ?g/ml<em> respectively which are in the same order as that of acarbose  (</em>123.26 ± 15.56 ?g/ml<em>) and significantly smaller (p < 0.0001) than that of the crude extract (</em>527.67 ± 16.32 ?g/ml<em>).</em></span></p> <p style="text-align: justify; line-height: 200%;"><span style="font-size: 14.0pt; line-height: 200%;">Conclusion:  The three fractions potently inhibited baker’s yeast alpha glucosidase, indicating presence of alpha glucosidase inhibitory molecules of diverse polarity and, hence, structures in the <em>T. catappa</em> stem bark extract. This investigation has suggested alpha glucosidase inhibition as a possible action mechanism of the anti-diabetic use of <em>T. catappa</em>, unmasking its stem bark extract as a repertoire for the discovery of compounds of diverse molecular structures with possible anti-diabetic alpha glucosidase inhibitory activities.</span></p>}, number={4}, journal={Nigerian Journal of Pharmaceutical and Applied Science Research}, author={Ajala, Olusegun and Makinde, Adeola and Ogah, Comfort}, year={2023}, month={Jan.}, pages={1–7} }